Expression and significance of P57(kip2) and Maspin in pathological scar

Objective To study the expression of P57kip2 and Maspin in the pathological scar and their possible role in the pathogenesis of abnormal scars.Methods Immunohistochemistry integrated image analysis and reverse transcriptionpolymerase chain reaction (RT-RCR) were performed to detect the expression of P57kip2 and Maspin in hypertrophic scar,keloid,mature scar and normal skin.Statistics was used to analyze the datas.Results The expression of P57kip2 protein was fixed to fibroblast intranuclear in abnormal scar,and the expression of P57kip2 protein and P57kip2 mRNA decreased ( P ＜ 0.05 ).The expression of Maspin protein was fixed to fibroblast cytoplasm and intranuclear in abnormal scar,and the expression of Maspin protein and Maspin mRNA decrease,compared with that in normal group ( P ＜0.05).There was positive correlation between P57kip2 protein and Maspin protein expression( P ＜ 0.01 ).Conclusions The decreased expression of P57kip2 and Maspin in abnormal scar shows that they are cicatrix-related genes.There is a positive relationship between the two genes.It may be one of the mechanisms of pathogenesis of abnormal scar.It makes effect through fibroblasts. Key words: P57kip2 ;  Maspin ;  Cicatrix, hypertrophic ;  Keloid