A facile transport assay for H+ coupled membrane transport using fluorescence probes
2012
A facile activity assay for an H+-coupled transporter using florescent probes was developed with an H+-coupled manganese transporter (MntH) as a model. Making use of coupled-proton transport, the transport activity (H+/Mn2+ cotransport) can be directly determined via fluorescence intensity changes of the probe, 5-(and-6)-carboxyfluorescein (5(6)-FAM). The approach of using highly sensitive fluorescence probes provides a more simple and convenient assay method for the determination of proton-coupled metal-ion uptake by transporters.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
35
References
5
Citations
NaN
KQI