The study of new SLA classical molecules in inbreeding Chinese Wuzhishan pig
2004
Abstract The pig may be used as an alternative organ donor source in the future. The Wuzhishan
miniature pig (WZSP) is a Chinese inbred mini pig with the highest inbreeding
coefficient and has been used in many biologic experiments.
We studied the classical MHC molecules of WZSP to confirm its pure gene
background and to provide information for xenotransplantation. Methods The classical class I (P1 and P14) and class II (DQA, DQB, DRA, and DRB) molecules were studied
using RT-PCR. The products were cloned into a pGEM-T vector, respectively, sequenced and compared with
related data for homology analysis. Results WZSP is highly homologous (>90%) with NIH miniature swine for class I
and class II molecules amino acids in α-3 domain responsible for the binding of human T-cell CD8 were largely
conserved; only two critical residues were altered. The critical residues of class I molecules recognized
by human natural killer (NK) cells were completely different from humans. Furthermore, new class II molecules were
homologous (>70%) with the Chinese south population in amino acids. The amino acids for
binding to human CD4 were identical in DRB and showed only two differences in DRA. Conclusions WZSP bears new alleles in porcine MHC-relevant loci. We might alter residues of class I
molecules to avoid killing by human NK cells. The striking similarities of DRB would make WZSP
less likely in compatible in xeno-rejection. We can also alter the two residues of the DRA
sequence to make WZSP a better model for xenotransplant research in China.
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