The Effect of All-trans Retinoic Acid in the Proliferation of Human Ovarian Cancer Cells HO8910

To study the effect of all-trans retinoic acid (ATRA) in the proliferation of human ovarian cancer cells, HO8910 human ovarian cancer cell lines were cultured in vitro and treated with different concentrations of all-trans retinoic acid for 1, 2 or 3 days.Cell proliferation was evaluated by MTT assay.Cell cycle was analayzed by flow cytometry (FCM).The expression of retinoic acid receptor α mRNA was detected by reverse transcription- polymerase chain reaction (RT-PCR).It is resulted that different concentrations of all-trans retinoicacid could inhibit the growth of HO8910 cells(P0.05) in a time and dose-dependent manner. All-trans retinoic acid increases the rates of G1 and decreases the rates of S and G2/M(P0.05), and therefore,it decreases cells proliferation index (PI﹡)(P0.05).It could up-regulate the expression of retinoic acid receptor α mRNA(P0.05),then inhibit proliferation of HO8910 cells.It is conclused that all-trans retinoic acid could inhibit the proliferation of human ovarian cancer cells HO8910,the molecular basis may be associated with the up-regulated mRNA of retinoic acid receptor α.