Quantitative analysis of trace elements in primary teeth by fluorescent X-ray spectrometer using APDC

Though fluorescent X-ray spectrometers have been applied for analysis of trace elements in many fields, few studies were reported on the odontological use of this method.In order to obtain a simple and quick analytical method for trace elements in deciduous teeth, pH of sample solution, APDC amount to be added, andaging time for precipitation were mainly examined.A deciduous tooth was dissolved by H NO3 and a metal complex was formed in which APDC bonded with trace elements in the tooth solution. Then, trace element analysis was carried out by a fluorescent X-ray spectrometer after the precipitation was collected on the Millipore filter.As a result, trace elements such as Cu, Zn, Pb, and Fe in primary teeth were determined quantitatively and it was elucidated that data were obtained without interference when excesses in the amounts of Ca and PO4 existing in teeth were removed, because the APDC did not form any precipitation with Ca2+and P8+.