Potential ability of different types of cyclodextrins to modulate the interaction between bovine serum albumin and 1-hydroxypyrene.
2020
Abstract Hydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) can bind with functional biomacromolecules and thus cause toxic effects in vivo. Four types of cyclodextrins (CDs) were selected to explore their potential ability to regulate the bindings between 1-hydroxypyrene (1-OHPyr) and bovine serum albumin (BSA) using multi-spectroscopic methods combined with molecular docking. The results showed that the four CDs caused varied modulating effects on the binding of BSA with 1-OHPyr, and the effects of γ-CD and (2-hydroxypropyl)-β-CD (HPCD) are the most significant. Specifically, γ-CD and HPCD could significantly reduce the binding affinity between 1-OHPyr and BSA, inhibit the micro-environmental changes of tryptophan residues, and slightly recover the helicity of BSA. The interactions and inclusion behavior of CDs with 1-OHPyr was the main reason why CDs could affect the binding of 1-OHPyr to BSA. The results indicated that γ-CD and HPCD might have potential application value in regulating the toxic effects of OH-PAHs.
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