Link between single nucleotide polymorphism of rs266729 and rs2241766 in the ADIPOQ gene and gestational diabetes in an Iranian population

Abstract Background The manuscript investigates the association between adiponectin gene (ADIPOQ) polymorphisms and gestational diabetes mellitus (GDM) in an Iranian population. Methods We designed a case-control study involving 265 normal glucose tolerant (NGT) women and 297 GDM patients. Three adiponectin single-nucleotide polymorphisms (rs1501299, rs266729 and rs2241766) were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and the protein level was measured by the enzyme-linked immunosorbent assay. Other biochemical parameters were assessed by conventional laboratory methods. Results We found that rs266729 and rs2241766 were significantly associated with GDM. The genotypes analysis indicated that for rs266729, 51(61.4%) of the 83 GDM patients had GG genotype, and 32(38.6%) GC/CC genotype and for rs2241766, 75(58.6%) of the 128 GDM patients had TT genotype, and 53(41.4%) GG/GT genotype. The relation between other single-nucleotide polymorphisms (rs1501299) and GDM was not observed. Conclusion The ADIPOQ genetic polymorphisms were associated with GDM in our studied population.