Positive effects of Forskolin (stimulator of lipolysis) treatment on cryosurvival of in vitro matured porcine oocytes

Abstract In order to examine its effect on oocyte lipid content and cryosurvival, Forskolin was added to the medium for in vitro maturation of porcine oocytes. Treatments were control (IVM without Forskolin during the 42 h incubation period), addition of 10 μM Forskolin for the entire 42 h (0–42) and addition of 10 μM Forskolin between 24 and 42 h only (24–42). In Experiment 1, treatments did not differ significantly in cleavage rate, but the blastocyst formation rate was lower in the 0–42 group than for control and 24–42 group oocytes (17, 32 and 40%, respectively; P 2 intensity of fluorescence, respectively; P in vitro matured porcine oocytes (Control group 28%, 0–42 h group 0%, 24–42 h group 26.67%). Addition of Forskolin affected the nuclear maturation of porcine oocytes. The percentage of PBE (polar body extrusion) were significantly reduced in the 0–42 h group (0–42 h group 42.00 ± 2.08 vs. Control group 79.70 ± 2.82 and 24–42 h group 70.60 ± 2.83; P in vitro -maturated porcine oocytes and that attendant chemical lipolysis did not impair their further development as it may have done in oocytes incubated with Forskolin for the full 42 h.