RAPD Profile Studies of Wheat Hybrids Obtained from Diallel Crossing

Ten random primers were used to study the suitability of RAPD technique for characterization of wheat hybrids obtained from diallel crossing and their parents. From this study, the patterns of molecular markers was classified into seven types including three categories: The first category included, Type I markers shared bands in both parents, and offspring; Type III markers shared bands in female parent and offspring; Type IV markers shared bands in male parent and offspring. The second category also included three types, Type II markers shared bands in male and female parents; Type V markers were presented in the female parent only; Type VI markers were present in the male parent only. Third category contained Type VII markers which were present in offspring only. The shared marker (category 1) ranged from 83.75% for hybrid number 2 (H2) to 92.66 % for hybrid number 3 (H3). While, the percentage of markers not shared in the studied hybrids (category 2) ranged from 5.33 % for hybrid number 1 to 15 % for hybrid number 5. The third category, type 7 marker, which identified a specific hybrid, did not appear in hybrids number H4, H5 and H6. While, unique markers related to the third category were observed in three crosses, which considered as a good marker to identifying new hybrids to protect the rights of plant breeders. However, RAPD markers are a powerful tool to detect different molecular markers in wheat hybrids.