Imaging β-galactosidase activity using 19F chemical shift imaging of LacZ gene-reporter molecule 2-fluoro-4-nitrophenol-β-d-galactopyranoside
2006
Abstract 2-Fluoro-4-nitrophenol-β- d -galactopyranoside (OFPNPG) belongs to a novel class of NMR active molecules (fluoroaryl-β- d -galactopyranosides), which are highly responsive to the action of β-galactosidase (β-gal). OFPNPG has a single 19 F peak (−55 ppm relative to aqueous sodium trifluoroacetate). Upon cleavage by β-gal, the pH sensitive aglycone 2-fluoro-4-nitrophenol (OFPNP) is observed at a chemical shift of −59 to −61 ppm. The chemical shift response is sufficient to observe β-gal activity using chemical shift imaging (CSI). 19 F CSI studies of enzyme activity and lacZ gene expression in 9L-glioma and MCF7 breast cancer cells are presented, providing further evidence for the utility of OFPNPG as a gene-reporter molecule for future in vivo studies.
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