Hiding in plain sight: Shellfish-killing phytoplankton in Washington State.

Abstract Summer bivalve shellfish mortalities have been observed in Puget Sound for nearly a century and attempts to understand and mitigate these losses have been only partially successful. Likewise, the understanding of the environmental conditions triggering shellfish mortalities and successful strategies for their mitigation are incomplete. In the literature, phytoplankton have played only a cursory role in summer shellfish mortalities in Washington State because spawning stress and bacteria were thought to be the primary causes. In recent years, the occurrence of Protoceratium reticulatum (Claparede & Lachmann) Buetschli and Akashiwo sanguinea (Hirasaka) Hansen & Moestrup, have been documented by the SoundToxins research and monitoring partnership in increasing numbers and duration and have been associated with declining shellfish health or mortality at various sites in Puget Sound. Blooms of these species occur primarily in summer months and have been shown to cause mass mortalities of shellfish in the U.S. and other parts of the world. In 2016-2017, yessotoxins (YTX) were measured in several species of Puget Sound bivalve shellfish, with a maximum concentration of 2.20 mg/kg in blue mussels, a value below the regulatory limit of 3.75 mg/kg established by the European Union for human health protection but documented to cause shellfish mortalities in other locations around the world. In July 2019, a bloom of P. reticulatum coincided with a summer shellfish mortality event, involving a dramatic surfacing of stressed, gaping Manila clams, suggesting that YTX could be the cause. YTX concentrations in their tissues were measured at a maximum of 0.28 mg/kg and histology of these clams demonstrated damage to digestive glands. A culture of P. reticulatum, isolated from North Bay during this massive bloom and shellfish mortality event, showed YTX reaching 26.6 pg/cell, the highest recorded toxin quota measured in the U.S. to date. Concentrations of YTX in phytoplankton samples reached a maximum of 920 ng/L during a P. reticulatum bloom in Mystery Bay on 13 August 2019 when cell abundance reached 1.82 million cells/L. The highest cellular YTX quota during that bloom that lasted into September was 10.8 pg/cell on 3 Sept 2019. Shellfish producers in Washington State have also noted shellfish larvae mortalities due to A. sanguinea passing through filtration intake systems into hatchery facilities. Early warning of shellfish-killing harmful algal bloom (HAB) presence in Puget Sound, through partnerships such as SoundToxins, provides options for shellfish growers to mitigate their effects through early harvest, movement of shellstock to upland facilities, or enhanced filtration at aquaculture facilities.