Detection of nematode-transmitted nepoviruses by the novel, one-tube AmpliDet RNA assay

A novel highly sensitive isothermal AmpliDet RNA system is described for specific detection of Arabis mosaic virus (ArMV), Raspberry ringspot virus (RpRSV), Strawberry latent ringspot virus (SLRSV), Tomato black ring virus (TBRV) and Tomato ringspot virus (ToRSV) in in vitro plant material. Viruses identified using a molecular beacon directed to a selected virus specific sequence within the amplicon formed during NASBA.