Callus induction on Aesculus chinensis and the determination of peroxidase isozyme

Plant growth regulators,carbon sources and sterilization time were studied on the callus induction from Aesculus chinensis young leaves as explant by orthogonal experimental.At the same time vertical polyacrylamide gel using electricity beach on POD calli was determined.The result showed that glucose was more conducive to the formation of leaf-induced calli from Aesculus chinensis than sucrose and maltose as a carbon source.The appropriate sterilization method was 0.1% HgCl2 soaking explants for 10 min.The optimal concentrations for callus induction was 2.4-D 2.0 mg/L and 6-BA 0.4 mg/L.Peroxidase isozyme bands increased significantly compared with the pre-inoculation of leaves and the difference in treatment between the different bands.