A new method to process testicular sperm: combining enzymatic digestion, accumulation of spermatozoa, and stimulation of motility

Purpose In azoospermia processing of the TESE material often results in a sample of reduced purity. This prospective study was set up to clarify whether a combination of enzymatic digestion, density gradient centrifugation and stimulation of motility (where indicated) is a feasible option in TESE patients.