Cloning and Expression of the Gene Coding for the Fusion Protein rhGM CSF/LIF

A fusion gene was constructed in a plasmid in which the coding regions of human GM CSF and LIF cDNAs were connected by a synthetic linker sequence encoding a short peptide G S G G S through DNA recombinant techniques. It was then subcloned into the pBV220 expression vector, and expressed in E.coli after transformation and temperature induction. The expressed protein named as rhGM LIF was confirmed by Western blot. After purification, the determination of activities showed that rhGM LIF exhibited both GM CSF and LIF activities.