Development of TLC and HPTLC Method for Determination ?-Mangostin in Mangosteen Peels (Garcinia Mangostana L.,)

The objective of this study is to investigate quantitative estimation of ?-mangostin in the fruit pericarp of Garcinia mangostana L. Five samples from mangosteen peels are named ethyl acetate extract, ethanol extract, ethyl acetate fraction, n-hexane fraction, and residue. Thin layer chromatography (TLC) method was developed for quantitative estimation of ?- mangostin in the five samples. Then, samples with the highest levels of ?-mangostin were tested by High Performance Thin Layer Chromatography (HPTLC) method. Ethyl acetate fraction gave the highest content of ?-mangostin. TLC method ?-mangostin content for ethyl acetate fraction, ethyl acetate extract, ethanol extract, n-hexane fraction, and residue were 33,61; 10,25; 8,73; 7,72; 7,50 % respectively. ?-mangostin content for ?-mangostin standard and ethyl acetate fraction with HPTLC method were 97,59; 34,17 % respectively. The result of the present study indicates that screening of extract and fraction of different solvents toward ?-mangostin shows ethyl acetate fraction as the choice for future development.