Localization of retinylphosphatase activity in subcellular fractions of rat liver

: Enzymic hydrolysis of retinyl phosphate in subcellular fractions of the rat liver has been investigated. A quantitative method of measuring activity of retinyl phosphatase has been developed. The method is based on the isolation of labeled retinol, the substance formed from chemically synthesized labeled retinyl phosphate through enzymatic hydrolysis. It has been shown that the major quantity of retinyl phosphatase activity (75%) with a specific activity of 80 micrograms retinyl phosphate hydrolyzed for 30 min/mg protein at 30 degrees C occurs in plasmatic membranes.