Effect of Xuebijing on cytokines HMGB-1 and TGF-β1 secreted by rat peritoneal mesothelial cells under the condition of high glucose.

Objective In this study,we investigated the effect of Xuebijing on the secretion of high mobility group box-1(HMGB-1)and transforming growth factor-β1(TGF-β1)in rat peritoneal mesothelial cells(PMC)under the condition of high glucose concentration,trying to understand the significance of Xuebijing on the prevention and treating of peritoneal fibrosis caused by peritoneal dialysis. Methods PMC were isolated from rat colic omentum,and the thirdly cultured PMC were used in the experiment. PMC were divided into experimental groups and control group at random. The experimental groups were treated with 2.5% glucose and Xuebijing of different concentrations. The super- nate was collected after 18 h,24 h and 36 h to detect HMGB-1 and TGF-β1 in it. Results The expression of HMGB-1 and TGF-β1 in all the experimental groups treated with 2.5% glucose was significantly higher than that in the control group (P 0.05).The expression of HMGB-1 and TGF-β1 in groups treated with 2.5% glucose and Xuebijing was significantly lower than that in groups treated with 2.5% glucose only (P 0.05);Xuebijing reduced the synthesis of HMGB-1 and TGF-β1 in a dose-dependent manner. Conclusion Glucose of high concentration can promote the synthesis of HMGB-1 and TGF-β1 in PMC. Xuebijing can reverse these changes and it may have significance in the prevention and treating of peritoneal fibrosis.