817: Prenatal prediction of neonatal intraventricular hemorrhage based on amniotic fluid markers

817 Prenatal prediction of neonatal intraventricular hemorrhage based on amniotic fluid markers Jose Bartha, Rocio Revello, Maria Alcaide, Daniel Abehsera, Sara Lopez-Magallon, Beatriz Herreros University Hospital La Paz, Division of Maternal and Fetal Medicine, Department of Obstetrics and Gynecology, Madrid, Spain, University Hospital La Paz, Clinical Chemistry, Madrid, Spain OBJECTIVE: To assess the predictive capability of some biological markers in the amniotic fluid of fetuses at high risk of neonatal intraventricular hemorrhage (IVH). STUDY DESIGN: Forty women at risk of very preterm delivery due to PPROM (n 1⁄4 29, 72.5%) or preterm labor with intact membranes and cervical length < 15 mm (n 1⁄4 11, 27.5%) were studied. Gestational age at study (diagnosis of the primary condition) was 26.91 2.59 weeks. Amniocentesis, universal and specific PCR and microbiological cultures were performed. Amniotic fluid IL18, IL 2, IL4, IL6, IL10, IL12, TNF-alpha, IFN-g and MMP-8 were measured by Multiplex method. Amniotic fluid glucose and leukocyte count were also measured by standard methods. Gestational age at study was also considered in the predictive models. Placental detailed histological studies were performed. Mann Whitney U test and forward stepwise binary logistic regression were used. RESULTS: In total 8 cases developed IVH (20%). Amniotic fluid concentrations of IL6, IL12, IL8, MMP-8 and leukocyte were significantly higher in those cases that develop IVH while gestational age at study was significantly lower. Binary logistic regression found 4 predictive models for IVH. The best of them (R2 1⁄4 0.69, p 1⁄4 0.0001) included in the model measurements of amniotic fluid IL6, IL12 and IL8 as well as gestational age at study. CONCLUSION: Clinical and biochemical markers of inflammation, specifically measurements of amniotic fluid IL6, IL12, IL8 and MMP-8, may predict the development of postnatal IVH in cases at high risk of this condition.