Study on isolation and systematic taxonomy of strains of genus Nesterenkonia

Four Gram-positive, Nesterenkonia-like strains were isolated from hypersaline soils collected from Xinjiang Province, China and East of Egypt. The four strains were studies using a polyphasic approach, which included morphological observation, growth pH and temperature rang, tolerance to NaCl,KCl,MgCl 2·6H 2O,CaCl 2, enzyme profiles, chemotaxonomy, (G+C) mol% content, 16S rDNA sequences analysis and DNA-DNA hybridization, and the only two type strains of the genus Nesterenkonia, Nesterenkonia halobia DSM 20541 T and Nesterenkonia lacusekhoensis DSM 12544 T were used as control. The test results showed the four isolates belonged the genus Nesterenkonia, but they had great differences from the other two valid species of the genus Nesterenkonia, and thus they should represent four new species of this genus.