[Protective effect of succinic acid on primary cardiomyocyte hypoxia/reoxygenation injury].

To establish cardiomyocyte hypoxia/reoxygenation injury model by culturing primary cardiomyocytes from suckling SD rats,in order to study the effect of succinic acid on LDH leakage rate cardiomyocyte ischemia / reperfusion injury. Furthermore,flow cytometry and western blot were conducted to detect the effect of succinic acid on cardiomyocyte apoptosis,cleaved caspase-3 and p-Akt,and discuss the protective effect of succinic acid on primary cardiomyocyte hypoxia / reoxygenation injury of primary cardiomyocytes from neonatal SD rats. According to the findings of the study,succinic acid at the concentrations ranging between 31. 25 mg· L- 1and 500 mg·L- 1had no significant effect on primary cardiomyocyte activity,and succinic acid at the concentrations of 400,200, 100,50 mg·L- 1could notably reduce cardiomyocyte ischemia / reperfusion LDH leakage rate( P 0. 01 or P 0. 05,respectively). Succinic acid at the concentrations of 400 mg·L- 1and 200 mg·L- 1could significantly reduce the percentage of cardiomyocyte apoptosis( P 0. 05),and inhibit the protein expression of cleaved caspase-3 caused by cardiomyocyte ischemia / reperfusion( P 0. 05). Succinic acid at the concentration of 400 mg·L- 1could remarkably increase the protein expression of cardiomyocyte Akt( P 0. 05), while succinic acid at the concentration of 200 mg·L- 1had no obvious effect on the protein expression of cardiomyocyte Akt. Therefore,this study demonstrated that succinic acid could inhibit necrosis and apoptosis caused by cardiomyocyte hypoxia / reoxygenation by activating Akt phosphorylation.