Acute regulation of Na+/H+ exchange, Na+:HCO3- cotransport, and C1-/base exchange in acid base disorders.

The aim of these experiments was to study acute regulation of proximal tubule H + /HCO 3 - transport systems in acid-base disorders. Proximal tubular suspensions were prepared from rabbit kidney cortex and incubated in acidic (pH 6.9), control (pH 7.d), or alkalotic (pH 8.0) media for 45 minutes and gassed with 5% CO 2 . Brush border membrane (BBM) and basolateral membrane (BLM) vesicles were isolated from the tubular suspensions and studied for the activity of the pH-regulating transport systems