Rolling Circle Amplification and Its Application in bio-molecular Diagnosis

Rolling circle amplification(RCA) is a new type of nucleic acid amplification technology developed in recent years.It is based on the amplification of a circularizable oligonucleotide probe(C-probe),which is ligated bv DNA ligase.Under isothermal condition,C-probe can be amplified and can generate multimeric single-stranded DNA(ssD-NA) complementary to C-probe.Compared with traditional nucleic acid amplification method,RCA is a simpler amplification method with high specificity.Combined with fluorescence detection,electrochemical detection,electrochemiluminescence detection,et al,RCA can be applied to high sensitive analysis of bio-molecules.Due to the fact that RCA can be carried out under constant temperature conditions,this feature make RCA hold great potential in the field of point-of-eare diagnosis.This article outlines the basic principles of RCA,the detailed description of the current status of rolling circle amplification in biological detection,and its future prospects.