抗日本脳炎ウイルス血清の2-Mercaptoethanol感受性に関する研究 (II)

Further studies were carried out to investigate the 2-mercaptoethanol (2ME)-sensitivity of JE virus antibody of pigs. The results obtained are as follows:1. In the natural infection cases, 2ME-sensitive antibodies were detected during the first 2 to 4 weeks of antibody response; thereafter the antibodies were found to be resistant to 2ME treatment.2. Five pigs have been subjected to the observation of 2ME-sensitivity of the sera for 2 or 3 consecutive years. Sera obtained from one pig at the third oversummering were found to be sensitive to 2ME treatment, i.e., the hemagglutination inhibition (HI) titers of the sera were reduced to a quarters of the original titers by 2ME treatment. However, the 2ME-sensitivity of antibodies of other cases which experienced the second or third oversummering, were found to be 2ME-resistant as before.3. In the experimental vaccination cases, the first antibodies produced after vaccination were found to be 2ME-sensitive and thereafter they became 2ME-resistant.4. Three pigs possessing 2ME-sensitive HI antibodies were detected in 173 cases possessing antibodies during the interepidemic period. Those were found in March, April and October in 1965, respectively.