Construction of a genetic linkage map in Lilium using a RIL mapping population based on SRAP marker
2015
A genetic linkage map of lily was constructed using RILs (recombinant inbred
lines) population of 180 individuals. This mapping population was developed
by crossing Raizan No.1 (Formolongo) and Gelria (Longiflomm) cultivars
through single-seed descent (SSD). SRAPs were generated by using restriction
enzymes EcoRI in combination with either MseI. The resulting products were
separated by electrophoresis on 6% denaturing polyacrylamide gel and
visualized by silver staining. The segregation of each marker and linkage
analysis was done using the program Mapmaker3.0. With 50 primer pairs, a
total of 189 parental polymorphic bands were detected and 78 were used for
mapping. The total map length was 2,135.5 cM consisted of 16 linkage groups.
The number of markers in the linkage groups varied from 1 to 12. The length
of linkage groups was range from 11.2 cM to 425.9 cM and mean marker interval
distance range from 9.4 cM to 345.4 cM individually. The mean marker interval
distance between markers was 27.4 cM. The map developed in the present study
was the first sequence-related amplified polymorphism markers map of lily
constructed with recombinant inbred lines, it could be used for genetic
mapping and molecular marker assisted breeding and quantitative trait locus
mapping of Lilium.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
18
References
3
Citations
NaN
KQI