Dual−Wavelength Ratiometric Electrochemiluminescence Immunosensor for Cardiac Troponin I Detection

Ratiometric electrochemiluminescence (ECL) has attracted special focus in the biological analysis field, because it could eliminate the environmental interference and allow for precise measurement. Herein, a dual-wavelength ratiometric ECL biosensor was designed for the detection of cardiac troponin I (cTnI), where (4,4′-dicarboxylic acid-2,2′-bipyridyl) ruthenium(II) (Ru(dcbpy)32+) and Au nanoparticle-loaded graphene oxide/polyethylenimine (GPRu–Au) nanomaterial acts as an acceptor, and Au nanoparticle-modified graphitic phase carbon nitride nanosheet composite (Au–CNN) acts as donor. Au–CNN shows a high and steady ECL signal centered at 455 nm, which is well-matched with the adsorption of GPRu–Au; thereby, a highly efficient electrochemiluminescent resonance energy transfer (ECL-RET) sensing platform is designed. AuNPs facilitate the immobilization of antibody on the nanomaterials through a Au–N bond. The high surface area of graphene oxide/polyethylenimine allows a large number of Ru(dcbpy)32+ to be lo...