Adaptation of a method for determining serum iron after deproteinization on a parallel analyzer

: The study of the determination of iron in sera by a bathophenanthroline method after deproteinization, has been realized according to the protocol Valtec conceived by SFBC, after adaptation on a FP9 parallel analyzer. The critical study of this adaptation included trials of within run precision (CV of 1.25%), total precision (CV 2.29 to 4.66%) as also evaluation of analytical range: the limit of linearity is 140 mumol/l. The evaluation of inaccuracy performed with patient specimens leads to establishment of follow up norms and interpretation norms of allometry line. Our whole results are in agreement with the performance standards of the protocol for the validation of methods published by the Societe Francaise de Biologie Clinique. Finally the described method is quick acting, reliable and very inexpensive.