북한강 수역에서 분리한 남조류 Anabaena circinalis 휴면포자 발아에 대한 환경요인의 영향

Akinete germination may be a starting point of some akinete-producing cyanobacterial blooms in eutrophic freshwater systems. This study examined germinability of akinete of a cyanobacterium Anabaena circinalis isolated from the sediment of the North Han River (Cheongpyung Dam), Korea, under several environmental factors such as temperature (5~25.C), light (0~100 μmol photons m-2 s-1), nutrients (nitrogen and phosphorus) and pH (5~12). The high germination rate appeared at high temperature: ¤55% at 25.C, followed by 15% at 15.C, 10.C and 10% at 5.C. Low light intensity was favorable for akinete germination. Over 45% of germination occurred at low light intensities (5, 15 and 30 μmol photons m-2 s-1), while less than 10% of germination occurred at both 50 and 100 μmol photons m-2 s-1. No germination occurred in the dark condition. Akinete germination rate increased with nutrient (phosphorus and nitrogen) enrichment, and nitrogen addition showed greater effect on the germination compared to phosphorus addition. Akinetes germinated well at neutral or slightly alkaline pH condition (pH 7 and pH 8: ¤55%), but no germination was observed at pH 11~12. The present study demonstrates some favorable ambient conditions of Anabaena circinalis germination, which could provide useful information to study the germination conditions of other Anabaena species or akinete-forming algae and predict its bloom in eutrophic freshwaters.