In Vitro Manipulation and Propagation of Gentiana L. Species from the Ukrainian Flora

Conditions were developed for microclonal propagation, callus induction and proliferation, plant regeneration, and long-term maintenance of fast-growing normal root cultures of Gentiana species from the Ukrainian flora. The basic growth parameters were evaluated for cultured tissues. Extensive growth and considerable biomass yield was achieved in most cultures. The ability to form tissue and organ cultures depended on the original genotype, type of explant, growth regulator, and mineral composition of the nutrient medium. The efficiency of regeneration from cultured tissue declined with the duration of callus maintenance.