Preparation of Rabbit Anti-goose IgG-HRP and Preliminary Application

To study on preparation of rabbit anti-goose IgG-HRP,the experimentation isolated IgG from the goose egg yolk,and the method was employed to dilute with water-n-octylic acid-ammonia sulfate initial extraction,out salt dialysis and DEAEA-50 chromatography.Protein nucleic acid detector and SDS-PAGE indicated the IgG concentration;the purified IgG was labeled with HRP;effect of GPV VP3 gene vaccine was detected with the labeled antibody.The results indicated that the initial extraction was 10 mg/mL and the purity was 94.5%;the antiserum characterized by immunodiffusion showed that the titer of the antiserum was 1∶32 and specificity of the antiserum was high.The titer of labeled antibody was 1∶3000;the labeled antibody was a good tool to detect the effect of GPV VP3 gene vaccine.