Effect of Cheongungdajosan-gamibang on oxidative damage and inflammation

ABSTRACT Objectives : The purpose of this study was verification of the anti-inflamm ation and anti-oxidant effect of Cheongungdajosan-gamibang extract (CG) in mouse macrophage, RAW 264.7 cells.Methods : We have basically using LPS-stimulated RAW 264.7 cells. The cell toxicity was determined by MTT assay. To evaluate the anti-inflammatory effect of Cheongungdaj osan -gamibang, amount of nitric oxide(NO) was measured using the NO detection kit and the IL-1β, IL-6 and TNF-α expression was measured by reverse transcriptase polymerase chain reaction (RT-PCR). Also, free ra dical scavenging assay has tested for DPPH and ABTS radical activity as well as the contents of total polyphenol.Results : In this study, 96.6% or higher cell viability was observed in all tested groups from 1, 10, 100 ㎍/㎖ in RAW 264.7 cells. The RAW 264.7 cells were induced by lipopolysaccharide (LPS) and CG 1, 10, 100 ㎍/㎖. The CG decreased nitric oxide (NO) production activity dose dependently, especially at 100 ㎍/㎖ of 55%. The production of IL-1β, IL-6 and TNF-α were decreased by 51%, 78% and 35% in CG treated 100 ㎍/㎖. CG showed dose-dependent suppression activity of reactive oxygen s pecies (ROS) production, especially at 100 ㎍/㎖ of 37%. DPPH radical scavenging activity and ABTS cation decolo rization were activated over 86% and 88% in CG at 1,000 ㎍/㎖ concentration.Conclusions : According to the results, we thought that CG showed anti-inflammatory and antioxidant activities on the RAW 264.7 cells in mouse macrophage. Therefore, this research is expected to provide the fundamental data about the natural material analysis of relating to the anti-inflammation and antioxidant.Key words : Cheongungdajosan-gamibang, Anti-oxidant, Anti-inflammatory, Fr ee radical, Cyotokine