Establishment of indirect ELISA for Type I duck hepatitis virus

TypeⅠduck hepatitis virus(DHV-Ⅰ) was proliferated with SPF chick embryos through the chick embryo allantoic sac inoculation.After multiple freeze-thaw cycles the collection of proliferated embryo allantoic fluid was purified through 0.22μm membrane filtration,differential centrifugation and sucrose density gradient centrifugation(SDGC),and then used as the ELISA coating antigen. And thus an indirect ELISA for DHV-Ⅰwas established.The comparative tests showed that this method has high sensitivity and good specificity and offers a convenient way to rapidly detect DHV- infection.