Microfluidic chip for biological chemiluminescence detection and detection method thereof

The invention discloses a microfluidic chip for biological chemiluminescence detection. The chip comprises an interface layer, a transparent layer, a channel layer, a reflecting layer and a fixed layer, which are sequentially arranged from top to bottom. The layers are connected by adhesives and fixed by fasteners; the interface layer and the transparent layer are respectively provided with a liquid inlet hole and a liquid outlet hole; the interface layer is also provided with a optical fiber interface; the channel layer is provided with a liquid inlet flow channel, a micro mixer, a detecting pool and a waste liquid buffer pool, which are successively communicate with each other; the optical fiber interface is arranged above the detecting pool; the bottom of the optical fiber interface is connected with a light penetration layer; the detecting pool is opened to the lower surface of the channel layer; the bottom of the detecting pool is connected with the reflecting layer; and the bottom of the waste liquid buffer pool is connected with the fixed layer. The invention also discloses a detection method of the microfluidic chip. The microfluidic chip improves reaction luminescence intensity of the detection solution and optical detection efficiency, and has advantages of simple preparation method, convenient operation, fast detection, high sensitivity, and accurate detection result.