The effect of vitamin D3 on nitric oxide levels in patients with multiple sclerosis

Background: Multiple sclerosis (MS) is a chronic, autoimmune disease of the central nervous system (CNS) of unknown etiology. Vitamin D3 (1,25(OH)2D3) has strong immune modulating potential. Nitric Oxide (NO) has been identified as one of the most destructive products of the immune system and is an important factor in demyelination. The effect of short-term vitamin D3 supplementation on NO level was assessed in MS patients. Materials and Methods: The study included 60 MS patients (male and female). Patients were randomized independently, in a double blind design, into one of two treatment groups. Controls (n=30) received current treatment. Vitamin D treated (n=30) individuals received current treatment plus 300000 IU vitamin D3. Vitamin D3 injection was repeated monthly for 6 months. Nitric oxide (NO) production was estimated by Griess reaction. Results: NO levels decreased following vitamin D3 treatment but the differences did not reach significance (12.411.1 µmol/L to 9.88.9 µmol/L). Control group was also associated with an