Capillary gas chromatographic—mass spectrometric determination of buspirone in plasma

Methods for determining buspirone plus a deuterated analogue and for buspirone alone in plasma samples are described. Analytes are prepared from plasma by liquid extraction into n-butyl chloride and subsequent back-extraction clean-up steps. Instrumental analysis involves selected-ion monitoring gas chromatography—mass spectrometry with fused-silica capillary chromatography. Quantification is in the range 0.05–10 ng/ml with acceptable accuracy and precision.