Lipid Peroxidation Studied by HPLC in Isolated Rat Liver: Comparative Efficiency of UW and HTK Preservation Solutions

Abstract Lipid peroxidation on preserved isolated rat liver. Lipoperoxidation (LPO) generated by activated oxygen species could be a cause of damage observed in isolated organs during normoxic reperfusion. Free and bound MDA, vitamin E and vitamin A analyses were performed by HPLC on extracts from livers preserved in two hepatic clinical preservation solutions; ATP level and intracellular pH were followed by 31P NMR. The data indicate that bound MDA values increased during ischemia and decreased upon cold and warm reperfusion. This rather unexpected MDA accumulation during ischemia supports the hypothesis that lipoperoxidation might be initiated by ADP-Fe+++-O2 − complex accumulating during this period. Furthermore, in lactobionate-solution preserved livers, we found during reperfusion a significant decrease in vitamin E content concomitantly with a decrease in bound MDA. This observation suggests a protective effect of vitamin E in lipoperoxide formation in livers stored in UW. This phenonenom is not obs...