Lipase-Catalyzed Synthesis of Diacylglycerol and Monoacylglycerol from Unsaturated Fatty Acid in Organic Solvent System

In the present paper lipase catalyzed synthesis of Diacylglycerol (DAG) and Monoacylglycerol (MAG) by esterification of glycerol and Oleic acid in an organic solvent was studied, and optimal conditions for acylglycerols synthesis by enzyme were established. Suitable biocatalysts were chosen on the basis of systematic screening experiments regarding their regio-selectivity, substrate tolerance. Lipases investigated were Rhizomucor miehei (RMIM) and Thermomyces lanuginosa (TLIM), which gave highest degree of esterification. The selective enzymatic production of Mono and Di-Olein (MO, DO) was optimized to obtain maximum yields. Hexane was useful organic solvent in glycerides synthesis. Based on this experiment, acylglycerols were synthesized on a preparative scale in good yields (>90%).