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Toxoplasma detection kit

2016 
The invention provides a monoclonal hybridoma cell strain 4D5.The collection number is CCTCC NO: C201624, the classification name is hybridoma cell strain No.4D5, and the collection date is February 1st, 2016.The invention further provides a toxoplasma detection kit.The toxoplasma detection kit is formed by a test strip, a standard sample, a reference sample and a sample diluent.An anti-toxoplasma SAG3 monoclonal monomer embedded in the test strip is secreted by the monoclonal hybridoma cell strain 4D5 with the collection number CCTCC NO: C201624.According to the toxoplasma detection kit, the immunochromatographic strip technology is adopted, and the technology is short in detection time, low in cost, easy to operate and suitable for field detection and epidemiological surveying; toxoplasma SAG3 serves as a diagnostic antigen; compared with SAG1 and SAG2, the expression quantity of the SAG3 is large, the toxoplasma SAG3 can be expressed in all infection stages of toxoplasma and more suitable for serving as the diagnostic antigen; the clinical application result shows that the toxoplasma detection kit is high in sensibility and specificity, good in stability and easy to popularize to the primary level.
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