Survey and Comparison of Sporecidal Property of CuO and AgO Nanoparticles upon Spore of Clostridium botulinum type E

Aims: In this paper examined sporecidal effect of metal oxide nanoparticles of silver and copper against bacterium Clostridium botulinum type E. Nanoparticles due to high volume levels are high sporecidal and bacterial properties. Methods: This study we used the bacterium Clostridium botulinum type E. and Silver and copper oxide nanoparticles with average diameter of 20 nm. All the colors and media culture used were of Merck Company. The bacterium determined and confirmed by PCR and antibody raised against Clostridium botulinum type E. The spores were performed with the Peterson method. Ratios of MIC / MBC and phenol Coefficient were calculated by Microdilution method and Syndics’ of sporecidal obtained amount of D value were determined by pure plate method. Results: Strains of bacteria confirmed by Catalytic domain of Clostridium botulinum type E. SEM and TEM images confirm nanoparticles size was about 20 nm. Power sporiecidal the nanoparticles were compared with other chemical sporecidale, like Glutaraldehyde. Phenol coefficient obtained of about 50 and the ratio MIC / MBC good was about 1/2, respectively. D value for the critical concentration of silver nanoparticles was about 7 minutes. Conclusion: The results of this paper showed that nanoparticles studied for its high ratio of surface to volume properties have high sporeidal and It is predicted can be used of this nanoparticles that the environment to by sterilize.