Biologic characteristics and chromosome karyotype analysis of lung adenocarcinoma cell strain A549/CDDP

Objective To create lung adenocarcinoma drug resistance cell strain A549/CDDP induced by cis-diaminodichloroplatin.Biologic characteristics and chromosome karyotype of cell strain were investigated to provide base to the studying of the mechanisms of lung adenocarcinoma.Methods Large dosage impact and step by step inducing method was used to create A549 drug resistance cell strain A549/CDDP.MTT was used to detect the cell drug resistance index.Bioluminescence was used to detect the energy metabolism.Cell cycle was analysized by flow cytometer.The technology of differential staining technique and SKY were used to analysis the chromatosome of A549/CDDP.Results The drug resistance index of A549/CDDP to seven drugs were different.The content of ATP,ADP and AMP decreased significantly.The cell cycle of A549/CDDP did not have notable changes.The results of the differential staining technique and SKY showed that the chromatosome of A549/CDDP had several derivative chromosomes.Conclusion CDDP can induce lung adenocarcinoma cell drug resistance strain A549/CDDP.The derivative chromosome of A549/CDDP may have relationship with the drug resistance.