Expression of the functional d-glucose transport system in Xenopus oocytes injected with mRNA of rat small intestine

Abstract mRNA prepared from rat small intestine was injected into Xenopus oocytes. The injected oocytes showed a clear electrical response to d -glucose (Glu) in the form of membrane depolarization and conductance increase, while none was shown to d -fructose. The membrane electrical response of the injected oocytes evoked by Glu followed the Michaelis-Menten type kinetics and was dependent on the membrane potential of the oocyte. Replacing the Na + of the bathing buffer with choline + resulted in no response to Glu. Thus, a Glu transport system coupled to a Na + gradient was expressed in Xenopus oocytes by injecting mRNA from rat small intestine.