Preservation of Dendrological Plants in the Southern Area of Romania

The natural reservations, public and private parks, green areas, from the Southern part of Romania are both providing a great source of dendrological biodiversity and an essential attribute of developments. These have a rather complex function – ecological role, equilibrium factor for the environment, social and health providing element for the society. Moreover, they are a soil preservation facilitation and a departure point for the evolution of plant biodiversity. Our professional team, directly involved in the research programs for the preservation of dendrological plants, established the following objectives : identification of dendrological species and varieties from the Southern area of Romania, selection of dendrological species and varieties with high adaptability to the typical soil and climate conditions and also with superior decorative traits, testing the behavior of selected dendrological species and varieties to multiplication through modern methods like in vitro techniques. In order, to archive these objectives we studied: 39 conifers species and varieties, 109 deciduous leaves species and varieties, 8 lianas varieties, 22 herbaceous species and 55 roses cultivars. Out of each group, between 5 to 50 dendrological species and varieties, were selected and tested in different culture environments using in vitro multiplication techniques next species: Thuja, Juniperus, Taxus, Chamaecyparis, Buxus, Eonymus, Callicarpa,Calicanthus, Kerria, Buddleja, Clematis, Vinca and others. In this work we presented in vitro multiplication of some conifers such as Thuja occidentalis var. aureum, Thuja occidentalis var. danica, Juniperus communis and Taxus baccata, using different variants of media culture and explant types. The aims of our experiments were to obtain the optimal concentration in growth regulators of culture media, which provide the best rate of multiplication.