STUDY ON THE GROWTH OF SYNERGIER IN THE SUPERNATANT OF BACILLUS THURINGIENSIS

The growth curves of synergier in the supernatant from strains of Bacillus thuringiensis GC-91.MP-342 and HD-1 were studied .The results showed that the production and accumulation of synergier began in the beginning of the logarithmic growth phase, and reached the top in the end of the logarithmic growth phase,and kept stable during the stable phase.Synergier in the supernatant from different Bacillus thuringiensis strains grew differently, strain GC-91 ( whose synergistic fold was 6) grew better than strain MP-342 ( whose synergistic fold was 3.4) , strain HD-1 grew badly ( whose synergistic fold was only 1.8); There was much similarity among the synergistic growth curve, crystal growth curve and bioaasay potency curve ,which indicated that the relations among synergier , crystal and bioaasay potency was remarkably positive correlation.Dissolved oxygen had some degree influence on the synergier growth ,abundant provision oxygen was contributed to the synergistic growth .