Effects of antibiotic residuals in dewatered sludge on the behavior of ammonia oxidizers during vermicomposting maturation process

Abstract Antibiotics existed in dewatered sludge may affect the organic decomposition and nitrification efficiency of vermicomposting process, and thus lowering the agricultural value of sludge vermicompost. However, few studies have focused on the effects of antibiotics during vermicomposting process of sludge, notably for the maturated phage. Hence, this study aimed to investigate the effects of antibiotics on the nitrification rate and the features of ammonia oxidizing archaea (AOA) and bacteria (AOB) during vermicomposting maturated phage of sludge. The treatments including the additions of tetracycline and ofloxacin with high and low concentrations were compared with the control without adding antibiotics. The results showed the antibiotics enhanced the nitrification rate of 15.8%–42% in vermicomposting maturated phage compared with the counterpart, with a better stimulating effect in the low concentration of tetracycline. The population of amoA genes increased in antibiotic treatments with low concentrations but decreased in these with high concentrations. In addition, high through-put sequencing results revealed that the tetracycline had a stronger influence on the α and β diversities of AOA and AOB, relative to the ofloxacin. In contrast to the AOB, the AOA played a more important role in ammonia oxidization, in the presence of antibiotics. This study suggests that the antibiotics of dewatered sludge can strongly affect the ammonia oxidization process through modifying the numbers and community structures of AOA and AOB during vermicomposting and these effects are associated with the types and concentrations of antibiotics.