HPLC determination of three isoflavones and lovastatin in Xuezhikang capsules

Objective:To establish an HPLC method for the determination of daidzein,glycitein,genistein and lov- astatin in Xuezhikang capsules.Methods:An HPLC method was applied with a YMC-C_(18)(4.6mm×250 mm,5 μm)column by a gradient elution using acetonitrile(A)-0.1% phosphoric acid(B)as the mobile phase.The detective wavelength was 256 nm.Results:The linearity ranges were 13.6×10~(-3)-81.6×10~(-3)μg(r=1.0000), 8.0×10~(-3)-48.0×10~(-3)μg(r=1.0000),24.0×10~(-3)144.0×10~(-3)μg(r=1.0000),4.50-8.25 μg(r= 0.9996)respectively.The average recoveries of daidzein,glycitein,genistein and lovastatin were 99.1%,98.5%, 98.2%,99.6% and RSD were 1.75%,1.37%,1.54%,1.49%(n=5).Conclusion:The method is accurate and sensitive.It is adoptable for quantitative analysis of daidzein,glycitein,genistein and lovastatin in Xuezhikang cap- sules simultaneously.