[Expression and Clinical Significance of Cancer-derived Immunoglobulin G in Non-small Cell Lung Cancer by Bioinformatics and Immunohistochemistry].

: 【中文题目：利用生物信息学、免疫组化分析肿瘤来源免疫球蛋白在非小细胞肺癌中的表达及临床意义】 【中文摘要：背景与目的 经典免疫学理论认为，免疫球蛋白G（immunoglobulin G, IgG）仅由B细胞合成。近年来研究发现恶性肿瘤细胞也可以合成IgG（cancer-IgG）。本研究分析了cancer-IgG在非小细胞肺癌（non-small cell lung cancer, NSCLC）中的表达及临床意义，并初步探究其机制。方法 应用数据库分析IgG1重链编码基因（immunoglobulin heavy constant gamma 1, IGHG1）、免疫组化分析cancer-IgG在NSCLC中的表达及与预后的关系；基因富集分析（gene set enrichment analysis, GSEA）方法探究与IGHG1调控相关的信号通路。结果 Cancer-IgG在NSCLC中的表达量显著高于正常组织，与预后呈负相关，并与患者的临床分期（P=0.042）、T分期（P=0.044）和转移（P=0.007）密切相关。GSEA分析显示，IGHG1与细胞黏附、细胞因子相互作用和趋化因子信号通路相关。结论 在NSCLC中，cancer-IgG高表达是预后不良的因素，可能与促进肿瘤的侵袭转移相关。】 【中文关键词：肺肿瘤；肿瘤来源免疫球蛋白；预后；机制】. METHODS: The expression of IgG1 heavy chain gamma 1 (IGHG1) and cancer-IgG were detected by bioinformatics and immunohistochemistry in NSCLC; The gene set enrichment analysis (GSEA) method was used to explore the signaling pathways involved in IGHG1 regulation. RESULTS: The expression level of cancer-IgG in NSCLC was significantly higher than that in normal tissues. The high expression group had a poor prognosis and was associated with clinical stage (P=0.042), T stage (P=0.044) and metastasis (P=0.007). GSEA analysis showed that IGHG1 was associated with cell adhesion, cytokine interaction and chemokine signaling pathway. CONCLUSIONS: High expression of cancer-IgG in NSCLC is a poor prognosis factor, which may be related to the promotion of tumor invasion and metastasis.