METHOD FOR DETERMINING THE LEVELS OF CARBONIC ANHYDRASE ISOZYMES IN INDIVIDUAL RED BLOOD CELLS

SUMMARY A procedure is presented which can be used to analyse the isozymes of carbonic anhydrase (CA I and CA II) as well as other specific soluble proteins of individual erythrocytes. The concentration of red cell CA I in the pig-tailed macaque (Macaca nemestrina) was estimated by the technique employed, as well as the relative concentration in individual cells. In addition, the basic technique was modified to include electrophoresis of the carbonic anhydrases of the individual cells.