Biological effects of targeted cell nuclear irradiation with monochromatic X-ray microbeams

Microbeams applied to radiobiological studies must be a powerful technique, especially examining non-targeted effects, such as bystander effects, genomic instability and radioadaptive response. However almost studies were carried out using high-LET helium-ion microbeams and limited experiments were available for examining biological effects induced by microbeams of electromagnetic radiations. Furthermore, the study for investigating non-targeted effects in cells irradiated with targeted nuclear or cytoplasmic irradiations was limited. In this study we have begun to investigate non-targeted cellular biological effects in normal human fibroblasts induced by targeted nuclear or cytoplasmic irradiations of low-LET X-ray microbeams.