High Throughput Protein Purification from Escherichia Coli for Functional Proteomics

TITLE and CONTENTS 1\. INTRODUCTION 5 1.1. High Throughput Experimentation 1.2. Proteomics 1.3. High Throughput Protein Purification 2\. OBJECTIVES AND STRATEGY 49 3\. METHODS 50 4\. RESULTS 53 4.1. Parallel Protein Production Scheme 4.2. Purification Chemistry for Functional Protein Production 4.3. High Throughput Protein Expression and Purification 4.4. Parameters Influencing Protein Purification Success 5\. DISCUSSION 86 5.1. Technical Development 5.2. Heterologous Protein Expression in Bacteria 5.3. Protein Production for Functional Proteomics 6\. REFERENCES 101 7\. APPENDIX 115 Abstract Zusammenfassung Abbreviations Publications Curriculum Vitae Acknowledgements