Tortoise shell and falsify random amplified polymorphism DNA (RAPD) appraisal method research

Purpose To establish a practical, fast and accurate identification method of tortoise shell, falsify amplified polymorphism DNA (RAPD) randomly and provide reliable basis for identification of tortoise shell. Method We use the Alkali cracking method to extract mitochondrial DNA from the tortoise shell and falsify and randomly do the amplification polymorphic DNA. Result The electrocardiogram of genuine and counterfeit goods tortoise shells mitochondrial DNA random amplified has obvious difference, the stripe number and location are all different, this method can effectively distinguish between genuine and counterfeit goods tortoise shells. Conclusion RAPD technology provides a simple, effective, high reliability for the identification of tortoise shell and its adulterants.